abstract：:The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian ...

journal_title：Journal of biotechnology

authors： de Winter L,Schepers LW,Cuaresma M,Barbosa MJ,Martens DE,Wijffels RH

更新日期：2014-10-10 00:00:00

abstract：:We previously developed a novel one-step biotransformation process for the production of α-ketoglutarate (α-KG) from L-glutamic acid by a Bacillus subtilis whole-cell biocatalyst expressing an L-amino acid deaminase (pm1) of Proteus mirabilis. However, the biotransformation efficiency of this process was low owing to ...

journal_title：Journal of biotechnology

authors： Hossain GS,Li J,Shin HD,Liu L,Wang M,Du G,Chen J

更新日期：2014-10-10 00:00:00

abstract：:Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the sa...

journal_title：Journal of biotechnology

authors： Bühligen F,Lindner P,Fetzer I,Stahl F,Scheper T,Harms H,Müller S

更新日期：2014-10-10 00:00:00

abstract：:Specific delivery of TNF-α antagonist to the inflamed site can increase its efficacy and reduce the side effects. In this study, we constructed a bispecific diabody (BsDb) that targets TNF-α and ED-B-containing fibronectin (B-FN), a fibronectin isoform specifically expressed in the pannus of the inflamed joint in rheu...

journal_title：Journal of biotechnology

authors： Liu M,Xie M,Jiang S,Liu G,Li L,Liu D,Yang X

更新日期：2014-09-30 00:00:00

abstract：:There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal...

journal_title：Journal of biotechnology

authors： Liu X,Liu C,Ma Y,Hong J,Zhang M

更新日期：2014-09-30 00:00:00

abstract：:The current validated endotoxin detection methods, in spite of being highly sensitive, present several drawbacks in terms of reproducibility, handling and cost. Therefore novel approaches are being carried out in the scientific community to overcome these difficulties. Remarkable efforts are focused on the development...

journal_title：Journal of biotechnology

authors： Mujika M,Zuzuarregui A,Sánchez-Gómez S,Martínez de Tejada G,Arana S,Pérez-Lorenzo E

更新日期：2014-09-30 00:00:00

abstract：:Signal peptides direct proteins to translocate across the bacterial cytoplasmic membrane. This study aimed to improve the level of extracellular secretion of recombinant carrageenase by recombining the gene encoding wild-type signal peptide (OmpZ) of Zobellia sp. ZM-2 κ-carrageenase into the expression vector pProEX-H...

journal_title：Journal of biotechnology

authors： Liu Z,Tian L,Chen Y,Mou H

更新日期：2014-09-20 00:00:00

abstract：:An O-methyltransferase SpOMT2884, originating from Streptomyces peucetius ATCC 27952, was cloned, expressed, and applied for the production of target metabolite from Escherichia coli. Biochemical characterization of the 25kDa recombinant protein by in vitro and in vivo experiments showed that SpOMT2884 was an S-adenos...

journal_title：Journal of biotechnology

authors： Koirala N,Pandey RP,Parajuli P,Jung HJ,Sohng JK

更新日期：2014-08-20 00:00:00

abstract：:A representative stoichiometric model is essential to perform metabolic flux analysis (MFA) using experimentally measured consumption (or production) rates as constraints. For Human Embryonic Kidney (HEK) cell culture, there is the opportunity to use an extremely well-curated and annotated human genome-scale model Rec...

journal_title：Journal of biotechnology

authors： Quek LE,Dietmair S,Hanscho M,Martínez VS,Borth N,Nielsen LK

更新日期：2014-08-20 00:00:00

abstract：:AL (SpA A domain-PpL B3 domain), LD5 (PpL B3 domain-SpA D domain-PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L-D-L) and LD3 (PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L) are novel evolved Ig binding molecules (NEIBMs) derived from the in vitro molecular evolution of combinatorial phage libraries displaying rando...

journal_title：Journal of biotechnology

authors： He T,Ding YY,Feng JJ,Chen QL,Zhu HM,Peng H,Rui B,Li XY,Cao MM,Pan W

更新日期：2014-08-20 00:00:00

abstract：:We report for the first time that the medicinal basidiomycete Lentinus edodes can reduce Au(III) from chloroauric acid (HAuCl4) to elemental Au [Au(0)], forming nanoparticles. Several methods, including transmission electron microscopy, electron energy loss spectroscopy, X-ray fluorescence, and dynamic light scatterin...

journal_title：Journal of biotechnology

authors： Vetchinkina EP,Loshchinina EA,Burov AM,Dykman LA,Nikitina VE

更新日期：2014-07-20 00:00:00

abstract：:The use of enzymes in organic solvents offers a great opportunity for the highly selective synthesis of complex organic compounds. In this study we investigate the POXylation of several enzymes with different polyoxazolines ranging from the hydrophilic poly(2-methyl-oxazoline) (PMOx) to the hydrophobic poly(2-heptyl-o...

journal_title：Journal of biotechnology

authors： Konieczny S,Krumm C,Doert D,Neufeld K,Tiller JC

更新日期：2014-07-10 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is the primary causative agent of an economically important swine disease, now known as porcine-associated disease (PCVAD). The only structural protein of viral capsid, Cap has become the major target for development of PCV2 subunit vaccines. The purpose of this study is to express Cap...

journal_title：Journal of biotechnology

authors： Chi JN,Wu CY,Chien MS,Wu PC,Wu CM,Huang C

更新日期：2014-07-10 00:00:00

abstract：:Based on the importance of heat shock proteins (HSPs) in diseases such as cancer, Alzheimer's disease or malaria, inhibitors of these chaperons are needed. Today's state-of-the-art techniques to identify HSP inhibitors are performed in microplate format, requiring large amounts of proteins and potential inhibitors. In...

journal_title：Journal of biotechnology

authors： Schax E,Walter JG,Märzhäuser H,Stahl F,Scheper T,Agard DA,Eichner S,Kirschning A,Zeilinger C

更新日期：2014-06-20 00:00:00

abstract：:Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

journal_title：Journal of biotechnology

authors： Straub M,Demler M,Weuster-Botz D,Dürre P

更新日期：2014-05-20 00:00:00

abstract：:Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the differenc...

journal_title：Journal of biotechnology

authors： Ding J,Zhang C,Gao M,Hou G,Liang K,Li C,Ni J,Li Z,Shi Z

更新日期：2014-05-10 00:00:00

abstract：:In concern with frequent recurrence of anthrax in endemic areas and inadvertent use of its spores as biological weapon, the development of an effective anthrax vaccine suitable for both human and veterinary needs is highly desirable. A simple oral delivery through expression in plant system could offer promising alter...

journal_title：Journal of biotechnology

authors： Gorantala J,Grover S,Rahi A,Chaudhary P,Rajwanshi R,Sarin NB,Bhatnagar R

更新日期：2014-04-20 00:00:00

abstract：:Gluconobacter oxydans can perform rapid incomplete oxidation of many sugars, sugar polyols and alcohols, and this outstanding ability shows a great potential in industrial bioconversion. Improvements of these industrially important strains would boost their productivities of important metabolites. However, the shortag...

journal_title：Journal of biotechnology

authors： Shi L,Li K,Zhang H,Liu X,Lin J,Wei D

更新日期：2014-04-10 00:00:00

abstract：:Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high ex...

journal_title：Journal of biotechnology

authors： Chen MX,Zheng SX,Yang YN,Xu C,Liu JS,Yang WD,Chye ML,Li HY

更新日期：2014-03-20 00:00:00

abstract：:The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR pathway in CHO cells was found to down-regulate the small nucleolar RNA U...

journal_title：Journal of biotechnology

authors： Courtes FC,Gu C,Wong NS,Dedon PC,Yap MG,Lee DY

更新日期：2014-03-20 00:00:00

abstract：:Bioprocesses such as production of organic acids or acid hydrolysis of bioresources during biofuel production often suffer limitations due to microbial sensitivity under acidic conditions. Approaches for improving the acid tolerance of these microbes have mainly focused on using metabolic engineering tools. Here, we t...

journal_title：Journal of biotechnology

authors： Basak S,Geng H,Jiang R

更新日期：2014-03-10 00:00:00

abstract：:Site selective chemical modification is a preferred method, employed to prolong the circulation half-life of biopharmaceuticals. Cysteines have been used as attachment point for such modification, however, to be susceptible for chemical modification the involved thiol must be in its reduced form. Proteins often contai...

journal_title：Journal of biotechnology

authors： Jespersen GR,Matthiesen F,Pedersen AK,Andersen HS,Kirsebom H,Nielsen AL

更新日期：2014-03-10 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

journal_title：Journal of biotechnology

authors： Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

更新日期：2014-02-10 00:00:00

abstract：:Melanins are predominantly indolic polymers which are having extensive applications in cosmetics, agriculture and medicine. In the present study, optimization of nutritional parameters influencing melanin production by Mucuna monosperma callus cultures was attempted using the response surface methodology (RSM). Standa...

journal_title：Journal of biotechnology

authors： Inamdar S,Joshi S,Bapat V,Jadhav J

更新日期：2014-01-20 00:00:00

abstract：:Previous studies demonstrated that hydrophobic proteins could be PEGylated in organic phase rather than water phase. It is still not known what the difference is for a hydrophilic protein's PEGylation in these two different phases. In this study, granulocyte colony stimulating factor (G-CSF) was dissolved in neat dime...

journal_title：Journal of biotechnology

authors： Peng F,Liu Y,Li X,Sun L,Zhao D,Wang Q,Ma G,Su Z

更新日期：2014-01-20 00:00:00

abstract：:The conversion of olive mill wastewater (OMW) into high added value lipids containing polyunsaturated fatty acids (PUFA), in parallel with a significant phenolic removal by selected strains of Zygomycetes, is reported here for the first time. The growth of Mortierella isabellina, Mortierella ramanniana, Cunninghamella...

journal_title：Journal of biotechnology

authors： Bellou S,Makri A,Sarris D,Michos K,Rentoumi P,Celik A,Papanikolaou S,Aggelis G

更新日期：2014-01-20 00:00:00

abstract：:Chlorella vulgaris was grown in two bench-scale photobioreactors with and without the application of a low intensity, low frequency electromagnetic field (EM-ELF) of about 3mT. Cell concentration and tendency of cells to form aggregates inside the reactor were recorded over a 30 days-time period at 0.5L-constant mediu...

journal_title：Journal of biotechnology

authors： Beruto DT,Lagazzo A,Frumento D,Converti A

更新日期：2014-01-01 00:00:00

abstract：:The expression of recombinant yeast pyruvate carboxylase (PYC) in animal cell lines was shown in previous studies to reduce significantly the formation of waste metabolites, although it has translated into mixed results in terms of improved cellular growth and productivity. In this work, we demonstrate that the unique...

journal_title：Journal of biotechnology

authors： Vallée C,Durocher Y,Henry O

更新日期：2014-01-01 00:00:00

abstract：:We developed an efficient chimeric promoter, MUASMSCP, with enhanced activity and salicylic acid (SA)/abscisic acid (ABA) inducibility, incorporating the upstream activation sequence (UAS) of Mirabilis mosaic virus full-length transcript (MUAS, -297 to -38) to the 5' end of Mirabilis mosaic virus sub-genomic transcrip...

journal_title：Journal of biotechnology

authors： Acharya S,Sengupta S,Patro S,Purohit S,Samal SK,Maiti IB,Dey N

更新日期：2014-01-01 00:00:00

abstract：:Most expression systems used for the over production of many enzymes employ carbon catabolite repressible promoters and hence must use sugar free, rich complex media. Use of expression systems to overcome carbon repression opens an avenue for exploiting cheap carbon sources for the production of recombinant enzymes. A...

journal_title：Journal of biotechnology

authors： Nathan S,Nair M

更新日期：2013-12-01 00:00:00

abstract：:A gene circuit for the controlled expression of a marker gene and for the assay of xylose concentration in Escherichia coli has been designed and tested. The xylF coding sequence for the xylose binding protein (XBP) was cloned in pT7T318U downstream from the promoter for xylanase A from B. subtilis (Pbsu), together wi...

journal_title：Journal of biotechnology

authors： Ribeiro LF,Bressan F,Furtado GP,Meireles F,Ward RJ

更新日期：2013-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are widely used for the production of biopharmaceuticals; however, our understanding of several physiological elements that contribute to productivity is limited. One of these is amino acid transport and how its limitation and/or regulation might affect productivity. To further our un...

journal_title：Journal of biotechnology

authors： Kyriakopoulos S,Polizzi KM,Kontoravdi C

更新日期：2013-12-01 00:00:00

abstract：:Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authent...

journal_title：Journal of biotechnology

authors： Skala W,Goettig P,Brandstetter H

更新日期：2013-12-01 00:00:00

abstract：:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In...

journal_title：Journal of biotechnology

authors： Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

更新日期：2013-12-01 00:00:00

abstract：:We investigated the growth kinetics and transcriptional responses of Corynebacterium glutamicum in environments with low (pCO2<40 mbar) and high (pCO2 ≥ 300 mbar) CO2/HCO3(-) levels compared to standard conditions. When cultivated at high CO2/HCO3(-)-levels, C. glutamicum showed increased (63%) biomass to substrate yi...

journal_title：Journal of biotechnology

authors： Blombach B,Buchholz J,Busche T,Kalinowski J,Takors R

更新日期：2013-12-01 00:00:00

abstract：:Exhaustive mutagenesis studies of the reactive centre loop (RCL), a key structural component of proteins belonging to the serpin superfamily of protease inhibitors, are complicated by the size of the RCL, serpin conformational complexity, and, for most serpins, the lack of a serpin-dependent phenotype of expressing ce...

journal_title：Journal of biotechnology

authors： Bhakta V,Gierczak RF,Sheffield WP

更新日期：2013-12-01 00:00:00

abstract：:N-Acetylneuraminic acid, an important component of glycoconjugates with various biological functions, can be produced from N-acetyl-D-glucosamine (GlcNAc) and pyruvate using a one-pot, two-enzyme system consisting of N-acyl-D-glucosamine 2-epimerase (AGE) and N-acetylneuraminate lyase (NAL). In this system, the epimer...

journal_title：Journal of biotechnology

authors： Klermund L,Groher A,Castiglione K

更新日期：2013-11-01 00:00:00

abstract：:It is quite important to understand the basic principle embedded in the main metabolism for the interpretation of the fermentation data. For this, it may be useful to understand the regulation mechanism based on systems biology approach. In the present study, we considered the perturbation analysis together with compu...

journal_title：Journal of biotechnology

authors： Matsuoka Y,Shimizu K

更新日期：2013-10-20 00:00:00

abstract：:Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating ma...

journal_title：Journal of biotechnology

authors： Zheng M,Su Z,Ji X,Ma G,Wang P,Zhang S

更新日期：2013-10-20 00:00:00